CD19 CAR T cell preparation kit is composed of three parts: T cell activation and expansion unit, T cell purification unit and CD19 CAR gene transduction unit. The T cell activation and expansion unit consists of T cell basal medium and T cell activation reagent, which provides nutrients for T cell culture, and the T cell activation reagent provides T cell activation signals for T cell activation; the T cell purification unit consists of T cell sorting beads and columns, which is capable of obtaining high purity T cells from single blood collection or PBMC; The CD19 CAR gene transduction unit contains CD19 CAR T spot lentivirus and T cell transduction enhancer (Viral E-hancer B), which enables efficient integration of CD19 CAR sequence. This kit is easy to operate, and prepares <font face="Calibri">CD</font>19 <font face="Calibri">C</font>ar <font face="Calibri">T</font> cells with high expansion multiplicity and cell viability, CAR gene transduction efficiency and T cell purity.
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Application of CD19 CAR T Cell Preparation Kit<o:p></o:p>
Applicable to the preparation of CD19 CAR-T cells of peripheral blood and cord blood origin.<o:p></o:p>
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Process development stage: shorten the time and cost of early process development of cell therapy products, and better help customers advance the development of CD19 CAR T cell therapy productsd.<o:p></o:p>
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Data of CD19 CAR T Cell Preparation Kit<o:p></o:p>
Purity of T cells before and after sorting:<o:p></o:p>
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Cell viability and expansion:<o:p></o:p>
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T cell transduction efficiency:<o:p></o:p>
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